Maintenance of multicopy plasmid Clo DF13

✍ Scribed by Hakkaart, Marcel J. J. ;van Gemen, Bob ;Veltkamp, Eduard ;Nijkamp, H. John J.

• Book ID: 104748432
• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 333 KB
• Volume: 198
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00383022

✦ Synopsis

To elucidate the mechanisms that operate in plasmid maintenance, we analysed the stability of different combinations of Clo DF13 derivatives present in the same bacterial cell. From the data described in this paper we conclude: (i) each Clo DF13 plasmid molecule has an equal chance of colonizing daughter cells upon cell division, (ii) the Clo DF13 minimal replicon harbours functions involved in plasmid segregation and incompatibility, (iii) in the case of cells harbouring plasmid replicons which differ in size, the smaller plasmid is gradually lost from the cell population, (iv) in the case of cells habouring plasmid replicons which differ in copy number, the lower copy number plasmid is always lost from the cell population. The effect of plasmid copy number is dominant over the effect of plasmid size,