Magnetic particles for the separation and purification of nucleic acids
โ Scribed by Sonja Berensmeier
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 247 KB
- Volume
- 73
- Category
- Article
- ISSN
- 1432-0614
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Polyphosphates of chain lengths between 3 and 100 were coupled to porous zirconia particles and used for affinity chromatography of nucleic acids and proteins. It is demonstrated that single-stranded RNA and DNA are strongly retained on the polyphosphate-modified zirconia support, while double-stran
An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichiu co/i is described. The method is applicable to multiple samples of about 5 X IO" cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little dete