✦ LIBER ✦

Preparation and Use of Polyphosphate-Modified Zirconia for Purification of Nucleic Acids and Proteins

✍ Scribed by B. Lorenz; S. Marme; W.E.G. Muller; K. Unger; H.C. Schroder

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 711 KB
Volume: 216
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1994.1015

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✦ Synopsis

Polyphosphates of chain lengths between 3 and 100 were coupled to porous zirconia particles and used for affinity chromatography of nucleic acids and proteins. It is demonstrated that single-stranded RNA and DNA are strongly retained on the polyphosphate-modified zirconia support, while double-stranded DNA is only weakly bound if at all. Protein separation on this packing can be performed by applying both high-performance liquid chromatography and batch procedures. The results show that polyphosphate-modified zirconia is a useful affinity packing for immobilization and purification of biomacromolecules.

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