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An improved method for the purification of the nucleic acids from small amounts of Escherichia coli

✍ Scribed by Allen C. Rogerson

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
534 KB
Volume
65
Category
Article
ISSN
0003-2697

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✦ Synopsis

An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichiu co/i is described. The method is applicable to multiple samples of about 5 X IO" cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little detectable degradation or selective loss of RNA, and virtually all of the cellular-derived carbon in the final preparation is nucleic acid. 34

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