Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.
A new affinity column for the purification of thymidine kinase from Escherichia coli
โ Scribed by Wolfgang Rohde
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 150 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0003-2697
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We have partially purified active delta and epsilon subunits of the E. coli membranebound MgZf-ATPase (ECF1). Treating purified ECFl with 50% pyridine precipitates the major subunits (a, 0, and y ) of the enzyme, but the two minor subunits (6 and E ) , which are present in relatively small amounts,
A continuous, automated assay for the measurement of acetate kinase activity in Escherichia co/i is described. The assay system consists of three steps: continuous sonication, acetate kinase catalyzed production of ATP, and subsequent measurement of ATP concentration. Complete extraction of acetate