Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.
✦ LIBER ✦
Importance of 3′-Hydroxyl Group of the Nucleosides for the Reactivity of Thymidine Phosphorylase from Escherichia coli.
✍ Scribed by Akihiko Hatano; Aiko Harano; Masayuki Kirihara
- Publisher
- John Wiley and Sons
- Year
- 2006
- Weight
- 8 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0931-7597
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