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A one-step procedure for the purification of uridine phosphorylase from Escherichia coli

✍ Scribed by Alberto Vita; Giulio Magni

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 300 KB
Volume: 133
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90236-1

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✦ Synopsis

Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.

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