m-Calpain (calcium-activated neutral proteinase) in Alzheimer's disease brains
โ Scribed by Teruyuki Tsuji; Shun Shimohama; Jun Kimura; Katsuji Shimizu
- Book ID
- 117476754
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 99 KB
- Volume
- 248
- Category
- Article
- ISSN
- 0304-3940
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๐ SIMILAR VOLUMES
Calcium-activated neutral proteinase (calpain) activity was determined, including in cytosol and membrane fractions, in rat glioma C6 cell line. The p and m forms of calpain were separated by DEAE and phenylsepharose column chromatography and with removal of the endogenous inhibitor calpastatin. C6
The activity of calcium-activated neutral proteinase (mM CANP) was determined in subcellular fractions of rat brain. The CANP activity in whole homogenate and its membrane fractions including myelin was increased tenfold following treatment with Triton X-100. The majority of the activity (60%) was i