The activity of calcium-activated neutral proteinase (mM CANP) was determined in subcellular fractions of rat brain. The CANP activity in whole homogenate and its membrane fractions including myelin was increased tenfold following treatment with Triton X-100. The majority of the activity (60%) was i
Calcium-activated neutral proteinase (calpain) in rat brain during development: compartmentation and role in myelination
β Scribed by Arun K. Chakrabarti; Naren L. Banik; Denise C. Lobo; Elaine C. Terry; Edward L. Hogan
- Book ID
- 119102101
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 830 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0165-3806
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## Abstract Calciumβactivated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The ΞΌM and mM Ca^2+^ βsensitive (ΞΌβand mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the la