Calcium-Activated Neutral Proteinase (Calpain) System in Aging and Alzheimer's Disease
β Scribed by R. A. NIXON; K.-I. SAITO; F. GRYNSPAN; W. R. GRIFFIN; S. KATAYAMA; T. HONDA; P. S. MOHAN; T. B. SHEA; M. BEERMANN
- Book ID
- 119871067
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 894 KB
- Volume
- 747
- Category
- Article
- ISSN
- 0890-6564
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Calcium-activated neutral proteinase (calpain) activity was determined, including in cytosol and membrane fractions, in rat glioma C6 cell line. The p and m forms of calpain were separated by DEAE and phenylsepharose column chromatography and with removal of the endogenous inhibitor calpastatin. C6
## Abstract Calciumβactivated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The ΞΌM and mM Ca^2+^ βsensitive (ΞΌβand mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the la