𝔖 Bobbio Scriptorium
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Lysosomal enzyme activities and low density lipoprotein receptors in circulating mononuclear cells

✍ Scribed by K. Henze; A. Chait

Book ID
104687229
Publisher
Springer
Year
1981
Tongue
English
Weight
433 KB
Volume
20
Category
Article
ISSN
0012-186X

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✦ Synopsis

Methods for the measurement of the lysosomal enzymes acid cholesterol-ester hydrolase and N-acetyl-beta-glucosaminidase were adapted for use with freshly isolated circulating mononuclear cells. Activities of both these enzymes increased (to 259 and 147% of control values respectively) after 7 days of insulin therapy in diabetic subjects. Low density lipoprotein degradation by freshly isolated mononuclear cells increased simultaneously by 67% (p less than 0.05). The findings in this pilot study suggest [1] that circulating mononuclear cells can be used to evaluate the effect of metabolic changes in vivo on lysosomal enzymes and low density lipoprotein metabolism, and [2] that insulin can stimulate cellular lysosomal enzyme activity.

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