Lysis and reassembly of sonicated lecithin vesicles in the presence of triton X-100
✍ Scribed by A. Alonso; A. Villena; F.M. Goñi
- Book ID
- 115912048
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 425 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0014-5793
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📜 SIMILAR VOLUMES
The Lowry procedure has been modified for use in the presence of Triton X-100 (TX-loo) by the addition of 10% sodium dodecyl sulfate. The method is applicable to samples containing 40-120 pg protein.
During our studies of the polynucleotide phosphorylase of rat liver mitochondrial membranes (1,2), we have needed to assay phosphatase, pyrophosphatase, and nucleoside diphosphatase activities in the presence of Triton X-100 used (2) to solubilize the membrane-bound polynucleotide phosphorylase. The
A procedure is described for the precipitation of protein from detergent-treated fractions of mammalian tissues. The protein in Triton X-100 and deoxycholatetreated fractions is precipitated by addition of sodium dodecyl sulfate in amounts nearly equivalent to that of the detergents, followed by tri