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Acid precipitation of protein in the presence of Triton X-100 and deoxycholate

โœ Scribed by Konrad C. Retz; William J. Steele

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
342 KB
Volume
79
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure is described for the precipitation of protein from detergent-treated fractions of mammalian tissues. The protein in Triton X-100 and deoxycholatetreated fractions is precipitated by addition of sodium dodecyl sulfate in amounts nearly equivalent to that of the detergents, followed by trichloroacetic acid to 5%, and is then collected by low-speed centrifugation. The recovery of protein is virtually 100%. Tissue protein precipitated in this way can be washed nearly free of labeled leucine. The method is convenient, inexpensive, and well-suited for the isolation of many samples.

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