During our studies of the polynucleotide phosphorylase of rat liver mitochondrial membranes (1,2), we have needed to assay phosphatase, pyrophosphatase, and nucleoside diphosphatase activities in the presence of Triton X-100 used (2) to solubilize the membrane-bound polynucleotide phosphorylase. The
Lowry determination of protein in the presence of Triton X-100
โ Scribed by Chi-Sun Wang; Robert L. Smith
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 169 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The Lowry procedure has been modified for use in the presence of Triton X-100 (TX-loo) by the addition of 10% sodium dodecyl sulfate. The method is applicable to samples containing 40-120 pg protein.
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A procedure is described for the precipitation of protein from detergent-treated fractions of mammalian tissues. The protein in Triton X-100 and deoxycholatetreated fractions is precipitated by addition of sodium dodecyl sulfate in amounts nearly equivalent to that of the detergents, followed by tri
A calorimetric method for the determination of orthophosphate in the presence of Triton X-100 and the extent of their mutual interference is presented. Effects of albumin and trichloroacetic acid on the reaction are also examined. The method is based on the very sensitive reaction developed for dete