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Ammonium molybdate-stannous chloride determination of orthophosphate in the presence of triton X-100

✍ Scribed by Steven D. Carson

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
310 KB
Volume
75
Category
Article
ISSN
0003-2697

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✦ Synopsis

A calorimetric method for the determination of orthophosphate in the presence of Triton X-100 and the extent of their mutual interference is presented. Effects of albumin and trichloroacetic acid on the reaction are also examined. The method is based on the very sensitive reaction developed for determination of orthophosphate by complex formation with ammonium molybdate followed by reduction with stannous chloride. The method allows determination of 0.005 pm01 of orthophosphate in the presence of up to 0.5% Triton X-100 and as little as 0.3% (v/v) Triton X-100 in the absence of phosphate.

Methods

Reagent stocks were prepared as follows. (a) Phosphate standard was prepared by dissolving K,HPO, (Fisher Scientific) in distilled water to make a 0.1 x 10m3 M solution.

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