โ Scribed by I. Loutfi; J. R. Batchelor; J. P. Lavender; A. A. Epenetos
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Monoclonal antibody 3E1.2 was coupled with the bifunctional chelate, cyclic DTPA anhydride, at molar ratios (cOTPAA:3E1.2) of 1:1, lO:l, 1OO:l and 1oOO:l. The desired substitution level of less than one mol of D"PA/bl of 3E1.2 was achieved at a molar ratio of 1O:l and a coupling efficiency of 9.9t3.
Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1 2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with ["qn]In acetate to a specific activity of 37-74kBq//~g (I
Polymeric paramagnetic ion chelate molecules were synthesized and covalently linked to monoclonal antibodies. The labeled antibodies retained their antigen binding capacity in vitro while carrying up to an average of 50 paramagnetic ion chelates, enabling specifically bound antibody concentrations l
An anti-carcinoembryonic antigen (CEA) antibody, NP-4, was labeled with 90Y using p-isothiocyanatobenzyl DTPA (SCN-Bz-DTPA) and its derivatives 1-(p-isothiocyanatobenzyl)-3-methyl-DTPA (1B3M), 2-(p-isothiocyanatobenzyl)-4-methyl-DTPA (1M3B), 1-(2)-methyl-4- isothiocyanatobenzyl-DTPA (MX-DTPA) as the