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Labelling of monoclonal antibodies 10B, 8C, and M2A with Indium-111

✍ Scribed by Raymond Reilly; Katherine Sheldon; Alexander Marks; Sylvain Houle

Publisher: Elsevier Science
Year: 1989
Weight: 305 KB
Volume: 40
Category: Article
ISSN: 0883-2889
DOI: 10.1016/0883-2889(89)90218-9

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✦ Synopsis

Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1 2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with ["qn]In acetate to a specific activity of 37-74kBq//~g (I 2 ~Ci/ttg). The radiochemical purity of the antibody preparation was 7681%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs.

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