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Lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (EBNA) in cord-blood lymphocytes infected by epstein-barr virus (EBV)

✍ Scribed by J. Yata; C. Desgranges; T. Nakagawa; M. C. Favre; G. De-Thé

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
558 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Human cord‐blood lymphocytes were infected with B95.8 Epstein‐Barr virus (EBV) before and after separation into B‐ and T‐cell populations. Lymphoblastoid cells exhibiting B‐cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated B‐cell subpopulation but not in the T‐cell subpopulation. EBV nuclear antigen (EBNA) was detected concurrently with the appearance of lymphoblastoid cells. The proportion of EBNA‐positive cells corresponded to that of lymphoblastoid cells, and reached 50% after 4 days. EBNA was present only in cells with B‐cell markers. These observations indicate that only B‐cells are susceptible to EBV infection, that the transformation occurs within a few days and that EBNA is a valid early marker for susceptibility to EBV transformation.

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