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Lowry determination of protein in the presence of sulfhydryl compounds or other reducing agents

✍ Scribed by Masashi Higuchi; Fumio Yoshida

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
342 KB
Volume
77
Category
Article
ISSN
0003-2697

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✦ Synopsis

The Lowry procedure has been modified for use in the presence of sulfhydryl compounds or other reducing agents by the addition of chloramine T under acid conditions before adding the alkaline-copper reagent. This method is applicable to the determination of 25-100 pg of protein, and a linear standard curve is obtained, though the sensitivity is slightly lower compared to that of the control.

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Lowry determination of protein in the presence of Triton X-100
✍ Chi-Sun Wang; Robert L. Smith πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 169 KB

The Lowry procedure has been modified for use in the presence of Triton X-100 (TX-loo) by the addition of 10% sodium dodecyl sulfate. The method is applicable to samples containing 40-120 pg protein.