## Abstract Lowβangle light scattering results reported previously demonstrated that measurements on high molecular weight native DNA must be made at angles below 30Β° in order to obtain correct molecular weights. Earlier lightβscattering data obtained on denaturated DNA at angles above 30Β° showed n
Low-angle light-scattering instrument for DNA solutions
β Scribed by J. A. Harpst; Alvin I. Krasna; B. H. Zimm
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1968
- Tongue
- English
- Weight
- 563 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0006-3525
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π SIMILAR VOLUMES
A very low-angle light-scattering photometer is described with respect to optical features, scattering cell, correction factors, and absolute calibration in the angular range 2'-35". An improved microfiltration apparatus was used to obtain essentially dust-free aqueous solutions for very low-angle l
## Abstract The molecular weight of native DNA is shown to decrease by at least a factor of two on denaturation by heat or alkali. This result is obtained only if lowβangle (<30Β°) lightβscattering measurements are used. Highβangle measurements (>30Β°) do not reveal a decrease in molecular weight on
A low-angle light-scattering instrument has been used to measure molecular weights of native calf-thymus and TT DNA. Molecular weights obtained by extrapolation of angular data to 0" from measurements above 30" are less than molecular weights from extrapolation of data taken at low angles (below 30"