Molecular weight of T7 and calf thymus DNA by low-angle light scattering

✍ Scribed by J. A. Harpst; Alvin I. Krasna; B. H. Zimm

• Publisher: Wiley (John Wiley & Sons)
• Year: 1968
• Tongue: English
• Weight: 498 KB
• Volume: 6
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1968.360060413

✦ Synopsis

A low-angle light-scattering instrument has been used to measure molecular weights of native calf-thymus and TT DNA. Molecular weights obtained by extrapolation of angular data to 0" from measurements above 30" are less than molecular weights from extrapolation of data taken at low angles (below 30"). The low-angle molecular weights determined for calf-thymus IIXA and for TT 11SA agree well with estimates of weightaverage molecular weight obtained with other techniques. The low-angle lightscattering molecular weight for calf-thymus DKA is higher than previously reported values by light scattering a t angles above 30". A ronrentration dependenre in the srnttering from DNA solutions is also observed.