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Low-angle light-scattering studies on alkali- and heat-denatured DNA

✍ Scribed by Alvin I. Krasna

Publisher: Wiley (John Wiley & Sons)
Year: 1970
Tongue: English
Weight: 549 KB
Volume: 9
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1970.360090906

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✦ Synopsis

Abstract

The molecular weight of native DNA is shown to decrease by at least a factor of two on denaturation by heat or alkali. This result is obtained only if low‐angle (<30°) light‐scattering measurements are used. High‐angle measurements (>30°) do not reveal a decrease in molecular weight on denaturation due to the incorrect value for native DNA. The dn/dc value for both native and denatured DNA is 0.166 ml/g ± 0.003 ml/g. Methods are described for the clarification of native and denatured DNA solutions for light scattering by the use of membrane filters.

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