LOCATION OF METASTATIC BREAST CARCINOMA BY A MONOCLONAL ANTIBODY CHELATE LABELLED WITH INDIUM-111
โ Scribed by Rainsbury, R.M.; Westwood, J.H.; Coombes, R.C.; Neville, A.M.; Ott, R.J.; Kalirai, T.S.; Mccready, V.R.; Gazet, J-C.
- Book ID
- 122646278
- Publisher
- The Lancet
- Year
- 1983
- Tongue
- English
- Weight
- 948 KB
- Volume
- 322
- Category
- Article
- ISSN
- 0140-6736
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๐ SIMILAR VOLUMES
Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1 2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with ["qn]In acetate to a specific activity of 37-74kBq//~g (I
Monoclonal antibody 3E1.2 was coupled with the bifunctional chelate, cyclic DTPA anhydride, at molar ratios (cOTPAA:3E1.2) of 1:1, lO:l, 1OO:l and 1oOO:l. The desired substitution level of less than one mol of D"PA/bl of 3E1.2 was achieved at a molar ratio of 1O:l and a coupling efficiency of 9.9t3.
Systemic administration of lysine to mice injected with indium-111-labelled Fab fragment of a monoclonal antibody has been shown to reduce renal uptake/retention of the radiometal. This treatment should be applicable to clinical immunoscintigraphy and radioimmunotherapy with radiometal-labelled anti