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Localized sensitivity enhanced in vivo 13C MRS to detect glucose metabolism in the mouse brain

✍ Scribed by C.I.H.C. Nabuurs; D.W.J. Klomp; A. Veltien; H.E. Kan; A. Heerschap

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
364 KB
Volume
59
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The application of in vivo ^13^C MR spectroscopy to mouse brain models is potentially valuable for improving the understanding of cerebral carbohydrate metabolism and glutamatergic neurotransmission in various neuropathologies. However, the low sensitivity of ^13^C nuclei and contaminating signals of lipids in the relatively small mouse brain make this application rather challenging. To meet these technical challenges, localized semi‐adiabatic distortionless enhanced polarization transfer (DEPT) MR spectroscopy in combination with a continuous intravenous [1,6‐^13^C~2~] glucose infusion was implemented to detect glucose metabolism in isoflurane‐anesthetized mice at 7T. The signal enhancement and high spectral resolution obtained in these experiments enabled the separate determination of ^13^C label incorporation into as much as 13 metabolites from a 175 μL volume. Signal increases of glucose (C6), glutamine (C3, C4), and glutamate (C3, C4) were determined with a time resolution of 8.6 min. This study demonstrates an optimized MR method for the application of in vivo ^13^C MRS in mouse brain. Magn Reson Med, 2008. © 2008 Wiley‐Liss, Inc.

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