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Localization of the Replication Region of the pMJ101 Plasmid from Vibrio ordalii

โœ Scribed by Carla Bidinost; Jorge H. Crosa; Luis A. Actis

Book ID
112263191
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
424 KB
Volume
31
Category
Article
ISSN
0147-619X

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A 1.3-kb segment of plasmid pSC101 includes the replication origin (ori) and the gene (rep) encoding the 37 kilodalton (K) protein required for autonomous replication of the plasmid. The present work describes the regulation of the rep gene expression. The gamma promoters PR and PL fail to promote r