The replication of plasmid pSC101
โ Scribed by D. Manen; L. Caro
- Book ID
- 110830700
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 337 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0950-382X
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๐ SIMILAR VOLUMES
A 1.3-kb segment of plasmid pSC101 includes the replication origin (ori) and the gene (rep) encoding the 37 kilodalton (K) protein required for autonomous replication of the plasmid. The present work describes the regulation of the rep gene expression. The gamma promoters PR and PL fail to promote r
The rate of replication of the plasmids colE1, pSC101, R100.1 and pAR132 (an RTF-TC derivative of the drug resistance factor R100.1) has been investigated directly by DNA:DNA hybridization. These rates have been compared, in a dnaAts strain, to that of various markers of the host chromosome at permi
Maintenance of plasmid pKC17, a derivative of plasmid pSC101, in E. coli requires a functional dnaA gene product. This was demonstrated by segregation experiments using an E. coli dnaA46 mutant, at various temperatures. Growth of this mutant at elevated temperature was allowed by the presence of a P