Localization of RecA in Escherichia coli K-12 using RecA–GFP
✍ Scribed by Nicholas Renzette; Nathan Gumlaw; Jared T. Nordman; Marlee Krieger; Su-Ping Yeh; Edward Long; Richard Centore; Ruethairat Boonsombat; Steven J. Sandler
- Book ID
- 110832585
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 572 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0950-382X
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The expression of the Escherichia coli uvrD gene was studied with a uvrD::Mud(Aprlac) insertion mutant, The results indicate that it is inducible by DNA damaging agents in a recA + gene-dependent manner.
A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to dNA-damaging agents; unable to support growth of the feckless red gam mutant lamb
The tif-1 mutation has been shown to affect protein synthesis in vitro by increasing translational ambiguity (Ephrati-Elizur, Luther-Davies and Hayes, 1976). It is demonstrated here that some recA mutations confer similar abnormality. By comparing suitable combinations of ribosomes and soluble prote