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Ribosomal abnormality in recA mutants of Escherichia coli

✍ Scribed by Powell, Keith A. ;Emmerson, Peter T.

Publisher: Springer
Year: 1977
Tongue: English
Weight: 374 KB
Volume: 154
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00265580

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✦ Synopsis

The tif-1 mutation has been shown to affect protein synthesis in vitro by increasing translational ambiguity (Ephrati-Elizur, Luther-Davies and Hayes, 1976). It is demonstrated here that some recA mutations confer similar abnormality. By comparing suitable combinations of ribosomes and soluble proteins from recA+ and recA cells the defect is shown to be associated with ribosomes. The recA mutation, which suppresses most phenotype characteristics of the tif-1 mutation (Castellazzi, George and Buttin, 1972(b)) does not suppress the ribosomal abnormality. Since the closely linked tif-1 and recA mutations lead to the expression of a common property they may be in the same gene.

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