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Localization of lactate dehydrogenase activity in the cells of the fish (Xiphophorus helleri) eye

✍ Scribed by Whitt, Gregory S. ;Booth, Gary M.


Publisher
John Wiley and Sons
Year
1970
Tongue
English
Weight
1000 KB
Volume
174
Category
Article
ISSN
0022-104X

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✦ Synopsis


The swordtail (Xiphophorus helleri) eye contains the Ad and B4 isozymes of lactate dehydrogenase (LDH) as do all vertebrates and in addition contains a distinctive lactate dehydrogenase, the Eq isozyme, found only in fish. LDH in the fish eye is located predominantly in the neural retina, especially in the inner segments of the photoreceptor cells. This neural retinal LDH activity consists mainly of the B4 and Eq isozymes which are more resistant than the Aq isozyme to inhibition by 1.8 M urea. The sclera, cornea, and lens possess mainly the urea-sensitive A4 isozyme. The specific cellular location of the Eq isozyme suggests that it plays a role in the bicchemistry of vision.


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