Localization of amplified MYC gene sequences to double minute chromosomes in acute myelogenous leukemia

✍ Scribed by Dr. Marilyn L. Slovak; Jennifer Pelkey Ho; Mark J. Pettenati; Aziz Khan; Daniel Douer; Shail Lal; S. Thomas Traweek

• Book ID: 102845503
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 506 KB
• Volume: 9
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870090111

✦ Synopsis

Cytogenetic and molecular studies were performed on two dmin-bearing acute myelogenous leukemia (FAB-M2) samples. Both cases were characterized by complex karyotypes containing interstitial deletions of the long arm of chromosome 8 altering band 8q24. I, aberrations affecting the short arm of chromosome 17, and multiple double minute chromosomes (dmin). Using a I .4 kb cDNA probe coding for the third exon of the MYC oncogene, D N A slot blots indicated MYC gene sequences were amplified in both samples. Fluorescence in situ hybridization using a 9.0 kb genomic probe for MYC was performed in one w e and localized the amplified MY& gene sequences to the dmin. Neither patient achieved a complete remission using traditional induction chemotherapy. The complex karyology with amplification of MYC gene sequences appears to represent a poor prognostic subgroup of acute myelogenous leukemia. Genes Chrom Cancer 9x52-67 (1994). 0 I994 Wiley-Liss, Inc.