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Localization of acid phosphatase in limb tissue of the adult newt,Diemictylus viridescens

✍ Scribed by Schmidt, Anthony J.


Publisher
John Wiley and Sons
Year
1963
Tongue
English
Weight
832 KB
Volume
152
Category
Article
ISSN
0022-104X

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✦ Synopsis


Acid phosphatase has been demonstrated histochemically in several mammalian tissues since Gomori introduced his method in 1941. Although surprisingly little information has been obtained about acid phosphatase in adult Urodele tissues, Kambara ('55) has demonstrated phosphatase reactivity in the epidermis, dermis, subcutaneous glands, and subcutaneous muscle, as well as in the urogenital system (Kambara, '56a, '56b) of adult Triturus pyrrhogaster.

In our studies on the regenerating adult newt limb, we surveyed the normal tissues for acid phosphatase reactivity, applying both the lead nitrate method of Gomori ('50), and the azo dye technique devised by Burstone ('58). Observations were made on epidermis, connective tissues, subcutaneous glands, blood vessels, blood smears, nerves and striated muscle. The results of these observations are unique in several respects, and serve as a basis for further investigation.


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