Liquid chromatography/thermospray mass spectrometry of monosaccharides and their derivatives

✍ Scribed by Miklós Lipták; Zoltán Dinya; Ferenc J. Sztaricskai; György Litkei; Jáozsef Jekö

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 449 KB
• Volume: 27
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/oms.1210271120

✦ Synopsis

Abstract

Thermospray (TSP) mass spectrometry has proved to be a useful technique for analysing various highly polar compounds. The use of a quadrupole mass spectrometer equipped with a thermospray/plasmaspray interface in the analysis of more than 30 monosaccharides and sugar derivatives is described. A 1:1 mixture of methanol or acetonitrile and 0.1 M aqueous ammonium acetate as eluent at 1 cm^3^ min^−1^ affords the best results. The correct setting of the capillary tip temperature and repeller voltage was fundamental for the ionization of carbohydrates. The/optimum values of these parameters were 240–250°C and 220–240 V, respectively. The thermospray mass spectra of most carbohydrates exhibit strong [M + NH~4~]^+^ ions which provide molecular mass information. The underivatized and derivatized monosaccharides could be grouped according to the presence or absence and the relative abundances of [M + NH~4~ − H~2~O]^+^, [M + H]^+^ and [M + NH~4~]^+^ ions.