This paper discusses the possible advantages of thermospray liquid chromatography/tandem mass spectrometry LC/MS/MS for metabolic mapping. The technique was applied to the study of the metabolism of zolpidem, a new hypnotic with an imidazo-pyridine moiety. When compared to other chromatographic/mass
Applications of thermospray liquid chromatography/mass spectrometry
β Scribed by L. Baczynskyj; G. E. Bronson
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 228 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1076-5174
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β¦ Synopsis
Thermospray liquid chromatography/mass spectrometry using a double-focusing magnetic sector mass spectrometer has been studied. The quality of the thermospray mass spectra were often a function of the solvent mixture used, and use of gradients affected the quality of some thermospray mass spectra. High resolution mass measurements using peak matching gave excellent results for pure compounds and mixtures which did not have 13C isotope interferences. When 3C isotope interference was a problem high-performance liquid chromatographic separation together with magnetic scanning was used.
π SIMILAR VOLUMES
Reversed-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) with thermospray ionization has been developed to analyze seco-triterpene lactone type quassinoids. Good results were obtained using an isocractic HPLC method with a mobile phase of acetonitrile + water (65:35 v/v%) an