Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5-bisoprolol as the internal standard

✍ Scribed by Gang-yi Liu; Wei Wang; Jing-ying Jia; Chuan Lu; Yan-mei Liu; Meng-qi Zhang; Yun Liu; Shui-jun Li; Chen Yu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 569 KB
• Volume: 24
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1329

✦ Synopsis

Abstract

A simple, reliable and sensitive liquid chromatography tandem mass spectrometry (LC‐MS/MS) protocol was developed and validated for quantification of bisoprolol in human plasma. The sample was pretreated with a simple procedure of protein precipitation and an isotope‐labeled d5‐bisoprolol was used as internal standard. The chromatographic separation was performed on a Capcell Pak C~18~ MG III column (100 mm × 2.0 mm, 5 µm). The protonated ion of the analyte was detected in positive ionization by multiple reaction monitoring mode. The mass transition pairs of m/z 326.3 → 116.3 and m/z 331.3 → 121.3 were used to detect bisoprolol and the internal standard, respectively. Linearity, accuracy, precision, recovery, matrix effect, dilution test and stability were evaluated during method validation over the range of 0.5–100 ng/mL. The validated method was successfully applied to analyze human plasma samples in a bisoprolol bioavailability study. Copyright © 2009 John Wiley & Sons, Ltd.