Rapid and sensitive liquid chromatography–tandem mass spectrometry method for the estimation of nicorandil in human plasma

✍ Scribed by Jignesh Bhatt; Arvind Jangid; Bhavin Shah; Raghavendra Shetty; Sandeep Kambli; Gunta Subbaiah; Sadhana Singh

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 109 KB
• Volume: 20
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.608

✦ Synopsis

Abstract

A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for the estimation of nicorandil in human plasma. Nicorandil was extracted from human plasma using solid‐phase extraction technique. Imipramine was used as the internal standard. A Betasil C~18~ column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid‐phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at nanogram levels. The proposed method has been validated for a linear range of 1.0–500.0 ng/mL with a correlation coefficient of ≥0.9993. The intra‐run and inter‐run precision and accuracy was within 10.0%. The overall recovery for nicorandil was 63.81%. The total run time was just 3.0 min. Copyright © 2005 John Wiley & Sons, Ltd.