A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the estimation of amlodipine in human plasma

✍ Scribed by Jignesh Bhatt; Sadhana Singh; Gunta Subbaiah; Bhavin Shah; Sandeep Kambli; Suresh Ameta

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 217 KB
• Volume: 21
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.730

✦ Synopsis

Abstract

A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for the estimation of amlodipine in human plasma. Amlodipine was extracted from human plasma by using a solid‐phase extraction technique. Imipramine was used as the internal standard. A Hypersil BDS C~18~ column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid‐phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at sub‐nanogram levels. The proposed method has been validated for a linear range of 0.1–10.0 ng/mL with correlation coefficient ≥0.9990. The intrarun and interrun precision and accuracy were within 10.0%. The overall recovery for amlodipine was 63.67%. Total run time was 3.2 min only. Copyright © 2007 John Wiley & Sons, Ltd.