Liquid chromatography in pharmaceutical analysis IV: Determination of antispasmodic mixtures

✍ Scribed by I. L. Honigberg; J. T. Stewart; A. P. Smith; R. D. Plunkett; E. L. Justice

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 460 KB
• Volume: 64
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600640829

✦ Synopsis

Parameters associated with the separation of antianxiety-antispasmodic agents were investigated using high-pressure liquid chromatography. Eight widely prescribed drugs were studied. The compounds were chromatographed on reversed-phase octadecyltrichlorosilane (CIS) or diphenyldichlorosilane (phenyl) columns, using mixtures of absolute methanol and distilled water buffered with ammonium dihydrogen phosphate, ammonium acid phosphate, or ammonium carbonate. A mixture of phenobarbitalpropantheline bromide was selected to demonstrate the utility of the separation and quantification method. The mixture was chromatographed on a phenyl column, using absolute methanol-aqueous 1% ammonium dihydrogen phosphate (60:40) (pH 5.85) at a flow rate of 1.4 ml/min. Each determination can be achieved in approximately 15 min with an accuracy of 1-2%.

Keyphrases 0 Antispasmodic mixtures-high-pressure liquid Chromatographic analysis, method parameters 0 Antianxiety-antispasmodic agents-high-pressure liquid chromatographic analysis, method parameters 0 High-pressure liquid chromatographyanalysis, antispasmodic mixtures 2 A. H. obins. Richmond, Va.