High-performance liquid chromatography a
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Yoshihiko Watanabe; Kazuhiro Imai
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Article
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1981
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Elsevier Science
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English
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7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60ยฐC for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se