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Liquid chromatographic determination of amino and imino acids and thiols by postcolumn derivatization with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazole

โœ Scribed by Watanabe, Yoshihiko.; Imai, Kazuhiro.


Book ID
120246529
Publisher
American Chemical Society
Year
1983
Tongue
English
Weight
728 KB
Volume
55
Category
Article
ISSN
0003-2700

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High-performance liquid chromatography a
โœ Yoshihiko Watanabe; Kazuhiro Imai ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 139 KB

7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60ยฐC for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se

Suppression of thiol exchange reaction i