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Fluorometric assay of amino acids and amines by use of 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole(NBD-F) in high-performance liquid chromatography

โœ Scribed by K. Imai; Y. Watanaba; T. Toyo'oka


Book ID
112719325
Publisher
Springer
Year
1982
Tongue
English
Weight
159 KB
Volume
16
Category
Article
ISSN
0009-5893

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๐Ÿ“œ SIMILAR VOLUMES


High-performance liquid chromatography a
โœ Yoshihiko Watanabe; Kazuhiro Imai ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 139 KB

7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60ยฐC for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se

HPLC electrochemical fluorometric detect
โœ Noriyuki Watanabe; Toshimasa Toyo'oka; Kazuhiro Imai ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 494 KB

It has been shown that by electrochemical oxidation 7-nitrobenzo-2-oxa-1,3-diazole-tryptophan (NBD-T) is converted to fluorophores having the same emission and excitation spectra as those for other NBD-amino acids. NBD-dioxindolylalanine was tentatively assumed to be a main electrochemical oxidation