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HPLC electrochemical fluorometric detection of amino acids including tryptophan using 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole

✍ Scribed by Noriyuki Watanabe; Toshimasa Toyo'oka; Kazuhiro Imai

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 494 KB
Volume: 2
Category: Article
ISSN: 0269-3879
DOI: 10.1002/bmc.1130020304

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✦ Synopsis

It has been shown that by electrochemical oxidation 7-nitrobenzo-2-oxa-1,3-diazole-tryptophan (NBD-T) is converted to fluorophores having the same emission and excitation spectra as those for other NBD-amino acids. NBD-dioxindolylalanine was tentatively assumed to be a main electrochemical oxidation product of NBD-tryptophan. A coulochemical cell placed between an analytical column and a fluorometer showed no detrimental effect on the separation of NBD-amino acids by reversed phase HPLC. Highly sensitive fluorescence detection was achieved for amino and imino acids at 10-100 fmol levels. The detection limit for tryptophan was 50 fmol.

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