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Liposome-mediated DNA transfer into chicken primordial germ cells in vivo

✍ Scribed by M. Watanabe; M. Naito; E. Sasaki; Dr. Michiharu Sakurai; T. Kuwana; T. Oishi

Book ID
111662658
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
755 KB
Volume
38
Category
Article
ISSN
1040-452X

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1Β°C/min until the temperature reached -80Β°C; cells were then