Lipids Part D

✍ Scribed by John M. Lowenstein (Eds.)

• Publisher: Academic Press
• Year: 1981
• Tongue: English
• Leaves: 876
• Series: Methods in Enzymology 72
• Edition: 1
• Category: Library

✦ Synopsis

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences

✦ Table of Contents

Content:
Contributors to volume 72
Pages xi-xv

Preface
Page xvii
John M. Lowenstein

Volumes in series
Pages xix,xxi-xxvi

[1] Extraction of tissue lipids with a solvent of low toxicity Original Research Article
Pages 5-7
Norman S. Radin

[2] Separation of methyl esters of fatty acids by gas chromatography on capillary columns, including the separation of deuterated from nondeuterated fatty acids Original Research Article
Pages 8-20
George M. Patton, Stefanie Cann, Henri Brunengraber, John M. Lowenstein

[3] High-performance liquid chromatography of triglycerides Original Research Article
Pages 21-34
R.D. Plattner

[4] High-performance liquid chromatographic separations of phospholipids and phospholipid oxidation products Original Research Article
Pages 34-40
Ned A. Porter, Hugo Weenen

[5] Separation of S-acyl-CoA thioesters and related compounds by reversed-phase ion-pair chromatography Original Research Article
Pages 41-52
Fred C. Baker, David A. Schooley

[6] Separation of partialy deuterated methyl palmitate from nondeuterated methyl palmitate by high-pressure liquid chromatography Original Research Article
Pages 53-56
C.E. Colson, J.M. Lowenstein

[7] Lipid analysis by liquid chromatography—mass spectrometry Original Research Article
Pages 56-108
Orville S. Privett, Warren L. Erdahl

[8] Solid state nuclear magnetic resonance of lipid bilayers Original Research Article
Pages 108-174
Robert G. Griffin

[9] DEAE-Silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids Original Research Article
Pages 174-185
Samar K. Kundu

[10] Thin-layer chromatography of neutral glycosphingolipids and gangliosides Original Research Article
Pages 185-204
Samar K. Kundu

[11] Flame ionization detection applied to thin-layer chromatography on coated quartz rods Original Research Article
Pages 205-252
R.G. Ackman

[12] Structure determinations of unsaturated fatty acids by oxidative fission Original Research Article
Pages 253-276
R.G. Ackman, J-L. Sebedio, W.N. Ratnayake

[13] Measurement of carnitine and O-acylcarnitines Original Research Article
Pages 276-287
L.L. Bieber, L.M. Lewin

[14] Determinationof choline, phosphorylcholine, and betaine Original Research Article
Pages 287-292
Anthony J. Barak, Dean J. Tuma

[15] Total phosphorus determination by spectrophotometry Original Research Article
Pages 292-295
Alasdair M. Cook, Christian D. Daughton

[16] Protein determination in membrane and lipoprotein samples: Manual and automated procedures Original Research Article
Pages 296-303
Mary Ann K. Markwell, Suzanne M. Haas, N.E. Tolbert, L.L. Bieber

[17] Assay of fatty acid synthase using a bicyclic dione as substrate Original Research Article
Pages 303-306
Alan H. Ullman, Harold B. White III

[18] Spectrophotometric procedure for measuring mitochondrial ОІ-oxidation Original Research Article
Pages 306-314
Harald Osmundsen

[19] Assay of peroxisomal ОІ-oxidation of fatty acids Original Research Article
Pages 315-319
Paul B. Lazarow

[20] Continuous measurement of free fatty acid release from intact adipocytes by pH-stat titration Original Research Article
Pages 319-325
Nils Г–sten Nilsson, Per Belfrage

[21] Measurement of lipoprotein lipase and hepatic triglyceride lipase in human postheparin plasma Original Research Article
Pages 325-338
Marietta L. Baginsky

[22] Bioluminescent assay for lipase, phospholipase A₂, and phospholipase C Original Research Article
Pages 338-346
Shimon Ulitzur, Michael Heller

[23] Assay for phospholipase C Original Research Article
Pages 347-351
Edward L. Krug, Claudia Kent

[24] Assay of enzymes of lipid metabolism with colored and fluorescent derivatives of natural lipids Original Research Article
Pages 351-375
S. Gatt, Y. Barenholz, R. Goldberg, T. Dinur, G. Besley, Z. Leibovitz-Ben Gershon, J. Rosenthal, R.J. Desnick, E.A. Devine, B. Shafit-Zagardo, F. Tsuruki

[25] Assay for lecithin: Cholesterol acyltransferase Original Research Article
Pages 375-384
Roy B. Verdery III, Shimon Gatt

[26] A novel assay method for the biosynthesis of galactosyl- and glucosylceramides: EC 2.4.1.45 UDPgalactose: 2-2-hydroxyacylsphingosine galactosyltransferase EC 2.4.1.80 UDPglucose: N-acylsphingosine glucosyltransferase Original Research Article
Pages 384-391
E. Costantino-Ceccarini, A. Cestelli

[27] Preparative enzymatic synthesis of acyl-acyl carrier protein Original Research Article
Pages 397-403
Charles O. Rock, Jeffrey L. Garwin, John E. Cronan Jr.

[28] Synthesis of long-chain fatty acyl-CoA thioesters using N-hydroxysuccinimide esters Original Research Article
Pages 404-408
Melvin Blecher

[29] Synthesis of modified fatty acids and glycerophospholipid analogs Original Research Article
Pages 408-433
Ramachandran Radhakrishnan, Robert J. Robson, Yohtaroh Takagaki, H. Gobind Khorana

[30] Preparation of long-chain hydroxyacyl thioesters: 3-hydroxyoctadecanoyl-CoA Original Research Article
Pages 434-435
Enzo Santaniello

[31] 12-Hydroxyeicosatetraenoic acid and related compounds Original Research Article
Pages 435-442
Frank F. Sun

[32] Chemical synthesis of furanoid fatty acids Original Research Article
Pages 443-471
Marcel S.F. Lie Ken Jie

[33] Pyrenedecanoic acid and pyrene lecithin Original Research Article
Pages 471-479
Hans-Joachim Galla, Wolfgang Hartmann

[34] Parinaric acid from Parinarium glaberrimum Original Research Article
Pages 479-482
Larry A. Sklar, Bruce S. Hudson, Robert D. Simoni

[35] Preparation of parinaric acid derivatives Original Research Article
Pages 483-485
Amy Tsai, Bruce S. Hudson, Robert D. Simoni

[36] Hydroxycitrate Original Research Article
Pages 486-497
John M. Lowenstein, Henri Brunengraber

[37] Agaric Acid Original Research Article
Pages 497-506
R.A. Freedland, Roger S. Newton

[38] Clofibrate Original Research Article
Pages 506-519
Jon Bremer, Harald Osmundsen, Renata Z. Christiansen, Borgar Borrebaek

[39] Cerulenin Original Research Article
Pages 520-532
Satoshi ЕЊmura

[40] 2-Tetradecylglycidic acid Original Research Article
Pages 533-551
G.F. Tutwiler, W. Ho, R.J. Mohrbacher

[41] 5-(Tetradecyloxy)-2-furoic acid Original Research Article
Pages 552-559
Robert A. Harris, Sylvia A. McCune

[42] 2-Bromooctanoate Original Research Article
Pages 559-577
Bruce M. Raaka, John M. Lowenstein

[43] 2-Bromoacetyl-SCoA Original Research Article
Pages 578-579
Peter R. Clements, John C. Wallace, D. Bruce Keech

[44] S-(4-Bromo-2,3-dioxobutyl)-CoA: An affinity label designed for acyl-CoA sites Original Research Article
Pages 580-583
Roland E. Barden, Martha S. Owens, Peter R. Clements

[45] Methoxycarbonyl-CoA disulfide: An active site=directed sulfhydryl reagent Original Research Article
Pages 584-587
Louis B. Hersh, Daniel J. Smith

[46] Affinity labeling with П‰-bromoacetamido fatty acids and analogs Original Research Article
Pages 587-591
Bryce V. Plapp, Wen-Sherng Chen

[47] Chloromethyl ketone derivatives of fatty acids Original Research Article
Pages 592-604
David P. Bloxham, R. Alan Chalkley, Gary Cooper

[48] 4-Pentenoic acid Original Research Article
Pages 604-610
Horst Schulz, Jim C. Fong

[49] Hypoglycin and metabolically related inhibitors Original Research Article
Pages 610-616
David Billington, H. Stanley, A. Sherratt

[50] 4-Pentenoylcarnitine, cyclopropanecarbonylcarnitine, and cyclobutanecarbonylcarnitine Original Research Article
Pages 616-619
Paul C. Holland, H.Stanley A. Sherratt

[51] Preparative isolation of lecithin Original Research Article
Pages 620-626
Norman S. Radin

[52] Extraction and purification of polyphosphoinositides Original Research Article
Pages 626-631
Jochen Schacht

[53] Preparation of phospholipids and their analogs by phospholipase D Original Research Article
Pages 632-639
Hansjörg Eibl, Stephan Kovatchev

[54] Synthesis of cyclopentanoid analogs of diacylglycerophosphate Original Research Article
Pages 640-672
Anthony J. Hancock

[55] Inhibitors of cerebroside metabolism Original Research Article
Pages 673-684
Norman s. Radin, Ranga Rao Vunnam

[56] 3-hydroxy-3-methylglutaryl-CoA reductase inhibitors from Penicillium and Monascus Species Original Research Article
Pages 684-689
Akira Endo

[57] Bacterial mutants for the study of lipid metabolism Original Research Article
Pages 693-707
David P. Clark, John E. Cronan Jr.

[58] Perifusion of fat cells Original Research Article
Pages 707-714
Donald O. Allen, Kathryn J. Long, Kasturi Sengupta

[59] Avian uropygial (preen) gland Original Research Article
Pages 714-720
P.E. Kolattukudy

[60] Growth and cytodifferentiation of 3T3-L1 preadipocytes into Adipocytes Original Research Article
Pages 720-723
Thomas R. Russell

[61] Preparation and culture of mammary gland explants Original Research Article
Pages 724-742
Raymond Dils, Isabel A. Forsyth

[62] Cultured avian liver explants for studies of lipogenic enzymes Original Research Article
Pages 743-747
Vasudev C. Joshi

[63] Isolation of plasma membranes from adipocytes Original Research Article
Pages 747-753
Douglas S. Lewis, Edward J. Masoro, Byung P. Yu

[64] Tissue culture of plants for studies of lipid metabolism Original Research Article
Pages 754-768
J.J. MacCarthy, P.K. Stumpf

[65] Polyunsaturation system from higher plants Original Research Article
Pages 768-773
Denis J. Murphy, P.K. Stumpf

[66] Preparation and use of protoplasts for studies of lipid metabolism Original Research Article
Pages 774-783
D.N. Kuhn, P.K. Stumpf

[67] Isolation and subfraction of glyoxysomes Original Research Article
Pages 783-790
Anthony H.C. Huang

Addendum to article [29]
Pages 793-794
Ramachandran Radhakrishnan, Robert J. Robson, Yohtaroh Takagaki, H. Gobind Khorana

Author index
Pages 795-821

Subject index
Pages 822-842