Light emission from bilirubin using the peroxyoxalate chemiluminescence reaction

✍ Scribed by Nian Wu; William J. Horvath; Carmen W. Huie

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 823 KB
• Volume: 269
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85138-v

✦ Synopsis

The observation of light emission from bdnubm m an orgamc solvent as a result of the peroxyoxalate chemdummescence reaction IS reported for the first time The results presented herem indicate that blhrubm chemdummescence can be detected m N,N' dlmethylformamlde but not m chloroform, dlmethylsulfoxlde, or pyndme To eluadate this phenomenon, the effects of solvent on the chemdummescence intensity were examined as a function of solvent composition Furthermore, analytical utlhzatlon of the present observation for the determmatlon of bduubm m biological materials was evaluated Relative and absolute hmlts of detection were found to be 8 5 nmol I-' and 5 0 ng, respectively, and the response was found to be linear at least over three orders of magnitude Selectlvlty for the detection of blbrubm m the presence of a number of common interference species was also examined Finally, the vlslble spectrum of bovme serum m the blue-green region was obtained using the present chemdummescence method and was briefly compared with those obtained from absorption ad fluorescence methods