𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Method for reducing the rate of light emission from chemiluminescent and bioluminescent reactions using frozen reagents

✍ Scribed by Kricka, Larry J. ;Ogden, Susan ;Williams, Thomas M. ;Kant, Jeffrey


Publisher
John Wiley and Sons
Year
1989
Weight
227 KB
Volume
3
Category
Article
ISSN
0884-3996

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✦ Synopsis


Frozen assay reagents have been used to reduce the rate of light emission from the rapid chemiluminescent acridinium ester and the bioluminescent firefly luciferase reactions. Melting of the assay reagent delays the initiation of the light emission, thus eliminating the need to initiate these rapid reactions by injection of the assay reagents in front of the photodetector.