A ligand-exchange chromatographic procedure for the selective separation of amino acids from inorganic ions is presented. It was found that the binding of amino acids to the nickel-Chelex 100 resin is pH dependent. At pH 8.5-9. I, only the basic amino acids lysine, histidine and arginine are quantit
Ligand-exchange chromatography of racemates : Resolution of α-amino acids
✍ Scribed by V.A. Davankov; S.V. Rogozhin; A.V. Semechkin
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 300 KB
- Volume
- 91
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Asymmetric resins containing optically active bi-or tridentate a-amino acids as fixed ligands coordinated with Cu 2+, NiZ+ or other transition metal ions can be used for the ligand-exchange chromatography of racemic a-amino acids. The degree of resolution of enantiomers depends both on the nature of the fixed and mobile ligands and on the metal ions employed.
📜 SIMILAR VOLUMES
Three chiral stationary phases, obtained by grafting silica gel with (-)-trans-l,2-~ycIohexanediamine, were studied for the resolution of a-amino acids by ligand-exchange chromatography.The packings were prepared by bonding thechiral ligand to silica gel via different hydrocarbon spacers. Separation