𝔖 Bobbio Scriptorium
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Life cycle of the moss,Physcomitrella patens, in culture

✍ Scribed by Paula A. Collier; Karen W. Hughes

Publisher
Springer
Year
1982
Tongue
English
Weight
376 KB
Volume
7
Category
Article
ISSN
1573-0603

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Spores have been preferred for mutagenic treatment of PhyscomitrelIa patens. Many mutant strains are, however, sexually sterile and so do not produce spores. We report here a method of somatic mutagenesis, followed by protoplast isolation, which allows a second round of mutagenesis in these mutants.

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By means of gas chromatography-selected ion monitoring-mass spectrometry using an isotope-dilution assay with 4,5,6,7-tetradeutero-indole-3-acetic acid as the internal standard, indole-3-acetic acid has been estimated to be present in aseptically cultured gametophytes of wild-type Physcomitrella pat