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Estimation of indole-3-acetic acid in gametophytes of the moss,Physcomitrella patens

✍ Scribed by Neil W. Ashton; Aga Schulze; Prudence Hall; Robert S. Bandurski

Publisher: Springer-Verlag
Year: 1985
Tongue: English
Weight: 270 KB
Volume: 164
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00391040

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✦ Synopsis

By means of gas chromatography-selected ion monitoring-mass spectrometry using an isotope-dilution assay with 4,5,6,7-tetradeutero-indole-3-acetic acid as the internal standard, indole-3-acetic acid has been estimated to be present in aseptically cultured gametophytes of wild-type Physcomitrella patens (Hedw.) B.S.G. at a level of 0.075 microgram g-1 dry weight or 2.1 ng g-1 fresh weight.

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