Somatic mutagenesis of the moss, Physcomitrella patens

✍ Scribed by Boyd, Philip J. ;Grimsley, Nigel H. ;Cove, David J.

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 192 KB
• Volume: 211
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425715

✦ Synopsis

Spores have been preferred for mutagenic treatment of PhyscomitrelIa patens. Many mutant strains are, however, sexually sterile and so do not produce spores. We report here a method of somatic mutagenesis, followed by protoplast isolation, which allows a second round of mutagenesis in these mutants. This method is also a convenient technique for routine isolation of mutants in wild-type P. patens.