Leukotriene B4 induces interleukin 5 generation from human T lymphocytes

✍ Scribed by Kunio A. Yamaoka; Jean-Pierre Kolb

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 950 KB
• Volume: 23
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830231003

✦ Synopsis

Leukotriene B4 (LTB4) has been shown to affect several interleukin (1L)-linked functions of human lymphocytes. In this study, we investigated whether LTBj regulates IL-5 generation from human T cells and subsequently modulates eosinophil functions. Preincubation of T cells with very low concentrations (10-l2 to M) of LTB4 induced concentration-dependent IL-5 production, the event occurring after the first 24 h of cultivation. However, direct action of LTB4 to IL-5 generation is strictly dependent on a preincubation with appropriate concentration of LTB4. In contrast, the stereoisomer of LTB4,5S,12S-dihydroxy-6,8,10,14eicosatetraenoic acid showed no enhancement of IL-5 production. IL-5 released from LTBj-primed T cells elicited sustained viability of mature eosinophils and reduced the content of eosinophil cationic protein in their crystalloid matrix by degranulation. These data suggest that LTBj induces bioactive IL-5 production from Tcells and that the released TL-5 modulates eosinophil functions which might play a crucial role in eosinophil-linked allergic inflammatory process.