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Laser nephelometry of lectin-glycoprotein complexes: A new method for their quantitation and differentiation

✍ Scribed by Eckart Köttgen; Christian Bauer; Wolfgang Gerok

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
320 KB
Volume
96
Category
Article
ISSN
0003-2697

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✦ Synopsis

The quantitative determination of glycoproteins and lectins by laser nephelometry is based on the fact that these two compounds form a complex comparable to the antigenantibody precipitate. Due to the high sensitivity and reliability of this technique less than 25 pmol of glycoprotein or 4.5 pmol of lectin can be determined, thus providing a suitable method (a) to investigate the specificity of different lectins for certain carbohydrate residues, (b) to study the mode of interaction, and (c) to explore mammalian tissue for still-unknown lectin-like substances.

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